A different sequencing method that chemically cleaved DNA at specific bases, developed by Allan Maxam and Walter Gilbert, was the dominant technology into the 1980s. Radiolabeled DNA samples were incubated in four separate reactions, each of which contained a chemical that cleaved after a different nucleotide — either A/G, G, C, or C/T. By adding the right amount of each chemical, it was possible to produce different fragments chopped off at each individual base. The sequence could then be read using gel electrophoresis and autoradiography. Maxam–Gilbert sequencing was easier than the plus and minus method to run and interpret, but was eventually surpassed by Sanger’s chain termination method, which molecular biologists found both technically preferable and more “elegant” since it mirrored the natural copying of DNA.
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For now, Asian economies can rely on their reserves, says Sung, who notes that Japan and South Korea have substantial oil stockpiles that can last over 200 days. China, too, has stockpiles amounting to three to four months of oil imports, while India’s supply can sustain the country for about two months.
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